Protein-polymer bioconjugates monomer

With both copolymerized fluorescent monomers the obtained fluorescent bioconjugates could be detected by SECrHPLC with fluorescence detection (at appropriate and for each fluorescent monomer) (Figure 5). This confirmed that the fluorescent probes had been incorporated in situ into the synthetic polymer-protein conjugate during the polymerization. In addition, SEC-FL-HPLC analysis revealed the absence of unreacted fluorescent monomers in the bioconjugate samples. 

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