Protein-polyelectrolyte complexes, factors

Earlier work (9) has shown that the size of the precipitate, but not the protein recovery, depends on the method of addition of the polymer to the protein solution. Mixing conditions in the precipitation vessel also affect the precipitate size (lOh The solubility of the protein-polyelectrolyte complex depends strongly on the solution conditions—pH, ionic strength, polymer dosage level, and the nature of the protein and polyelectrolyte. These factors are discussed below ... 

All of the states of protein-polyelectrolyte complexes referred to above may be achieved by the selection of the polyelectrolyte (PE), choice of ionic strength and pH, and control of the concentration of the macromolecular components. Most reports on the practical applications of protein-polyelectrolyte complexes concern the effects of these factors. This chapter focuses on the effects of polymer charge density, solution pH and ionic strength on protein-polyelectrolyte com-plexation discussed in recent and current research. Techniques employed in the study of protein-polyelectrolyte complexes and applications of the complex to protein separation and enzyme immobilization are also described. 

Factors Influencing Protein-Polyelectrolyte Complexation and Structures of the Protein-Polyelectrolyte Complexes... 

Factors influencing Protein-Polyelectrolyte Complexation 261 153,2 pH or Protein Charge... 

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