Protein, microwave absorbance

For instance, if ampules of aqueous products were to be sterilized in a hot air oven, the mechanisms of microbial inactivation would still be by coagulation of intracellular proteins. However, heat transfer from hot air is much slower than heat transfer from steam, which is why this is not seen as a practical process. Microwave irradiation could be an alternative means of sterilizing aqueous pharmaceutical products utilizing the same antimicrobial mechanisms as steam certainly there is evidence that microwave killing patterns are mainly due to heat transfer with very little direct energy being absorbed from the microwaves. 

MR provides a very simple and effective analytical method. Hydrolysis of peptides or proteins (may be microwave assisted) followed by derivatization of the resulting amino acids with the chiral MR adds a highly absorbing chromophore that converts the amino acids into UV-active diastereomers. This allows separation of D- and L-amino acids as diastereomers in the nanomole range on a nonchiral reversed phase standard column with the inherent rapidity of determination in HPLC. In addition, these derivatives of amino acids can be detected in both, simple UV as well as more selective mass spectrometric devices. 

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