Protein gold-labeled

FIGURE 1.2 In situ localization of the OCP-green fluorescence protein (GFP) fusion protein Immunogold labeling of a thin section of OCP-GFP transformed Synechocystis PCC6803 OCP-GFP cells were labeled with a polyclonal antibody against the GFP coupled to 10 nm gold particles. Bar = 0.5 pm. 

This section discusses the properties of gold particles as well as the common methods of labeling proteins and other biomolecules with them. The cited references should be consulted to obtain protocols for using these protein-gold complexes in assay and detection systems. 

The following sections discuss the preparation of colloidal gold suspensions of various particle sizes and their use in labeling proteins for detection purposes. Gold-labeled molecules and proteins are available from a number of manufacturers (Janssen, E-Y Labs, and Nanoprobes). 

Horisberger, M. (1989) Quantitative aspects of labeling colloidal gold with proteins, in Immuno-gold Labeling in Cell Biology (Verkleij, A. J. and Leunissen, J. L. M., eds.), CRC, Boca Raton, FL, pp. 49-60. 

Carbohydrates were cy to chemically detected in hydrogenosome membranes using the periodic acid-thiosemicarbazide-silver proteinate technique (Fig. 6a) and gold-labeled lectins, such as WGA (Fig. 6b) (Benchimol... 

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