Protein gels clear

Examine the gel and decide if it is stained dark enough to see the protein bands clearly. 

An interesting application of CRIE is the preparative aspect. The separation of proteins into clearly positioned and well-spaced precipitates provides an easy means of obtaining purified antigens for immunization of animals. By loading the gel maximally with a protein sample, there will be sufficient material for successful immunization. 

The data from these experiments did not give a clear-cut picture as to the origin of the extracellular amylase activity. Some autolysls did take place during the stationary phase, but the excretion of protein was clearly growth related. The liberation of glucose (Figures 1 and 2) Indicated that glucoamylase was part of the amylase activity. Preliminary experiments (unpublished) with gel electrophoresis have shown more than one protein with amylase activity. 

The large pore structure of the TSK-GEL G6000PW allows it to separate large molecules such as pBR322 plasmid from contaminating RNAs and proteins in a much shorter time frame than other methods (23). A two column system of G6000PW (7.5 mm i.d. X 60 cm) was used to separate the cleared lysate and phenol extract of the plasmid as shown in Fig. 4.34 (page 130). The plasmid... 

Definition of Ej and E2 eonformations of the a subunit of Na,K-ATPase involves identification of cleavage points in the protein as well as association of cleavage with different rates of inactivation of Na,K-ATPase and K-phosphatase activities [104,105]. In the Ei form of Na,K-ATPase the cleavage patterns of the two serine proteases are clearly distinct. Chymotrypsin cleaves at Leu (C3), Fig. 3A, and both Na,K-ATPase and K-phosphatase are inactivated in a monoexponential pattern [33,106]. Trypsin cleaves the E form rapidly at Lys ° (T2) and more slowly at Arg (T3) to produce the characteristie biphasic pattern of inactivation. Localization of these splits was determined by sequencing N-termini of fragments after isolation on high resolution gel filtration columns [107]. 

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