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Protein engineering, aromatic

Harford-Cross CF, Carmichael AB, Allan FK et al (2000) Protein engineering of cytochrome P450cam (CYP101) for the oxidation of polycyclic aromatic hydrocarbons. Protein Eng 13 121-128... [Pg.203]

TABLE 17.1 Examples of Protein Engineering Approaches Used to Improve or Modify Aromatic Hydrocarbon Dioxygenases... [Pg.460]

For the hydrophobic contribution, measurements of solvent transfer give AG values of 80-140 J/(mol Studies with engineered proteins indicate similar numbers, but in some cases, values as high as 500 J/(mol A ) are given. Experimental data from associations of about 50 different aromatic compounds, which are noticeably independent of heteroatoms and charges within the it-systems, lead to a value of 430 J/(mol A ). ° The size exclusion AA can be conveniently assessed by computer-aided evaluation of the surface difference for the host and guest molecules before and after association. [Pg.674]

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