Protein-coated microcrystals

Immobilized soybean peroxidase stability after ten washes and even after storage of the sample suspensions in a refrigerator (4°C) for 5 months The protein-coated microcrystals [20]... 

Kreiner M, Parker MC (2005) Protein-coated microcrystals for use in organic solvents application to oxidoreductases. Biotechnol Lett 27 1571-1577... 

The use of oxidoreductases in solution clearly dominates over immobilized applications. Use of immobilized whole cells (e.g. baker s yeast) [16] is, however, well described, and reports have also appeared claiming increased stability and activity of isolated horse liver alcohol dehydrogenase and other oxidoreductases immobilized on agarose [17] or salt crystals [18] (protein-coated microcrystals, PCMC [19]). Furthermore, immobilization of oxidoreductases on surfaces has been studied more intensively for the development of biosensors. 

There are several ways to coat a MALDI plate. One is two-layer overlayer [62,63] In this method, the first layer on the MALDI target plate comprises the densely packed matrix microcrystals formed by the quick solvent evaporation of a matrix solution in acetone or a mixture of acetonitrile and water at room temperature. A mixture solution containing both matrix and sample is then deposited onto the first layer of small crystals to form uniform analyte-matrix microcrystals. The overlayer method is reported to provide improved results, particularly for analyses of proteins and peptides [64-66],... 

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