Protein, carbonylic groups

Ahn, B., Rhee, S.G., and Stadtman, E.R. (1987) Use of fluorescein hydrazide and fluorescein thiosemicar-bazide reagents for the fluorometric determination of protein carbonyl groups and for the detection of oxidized proteins on polyacrylamide gels. Anal. Biocbem. 161, 245-257. 

Hipkiss, A. R. and Brownson, C. (2000). Carnosine reacts with protein carbonyl groups Another possible role for the anti-ageing peptide Biogerontology 1, 217-223. 

Hipkiss, A. R., Brownson, C., and Carrier, M. J. (2001). Carnosine, the anti-ageing, antioxidant dipeptide, may react with protein carbonyl groups. Mech. Ageing Dev. 122, 1431-1445. 

Morrissey 53) used transmission infrared spectroscopy to study protein adsorption onto silica particles in a heavy water (DzO) buffer. By observing the shift in the amide I absorption band, he could deduce the fraction of protein carbonyl groups involved in bonding to the silica surface. He found that bovine IgG had a bound fraction of 0.20 at low bulk solution concentrations, but only about 0.02 at high solution concentrations. However, neither prothrombin nor bovine serum albumin exhibited a change in bound fraction with concentration. Parallel experiments with flat silica plates using ellipsometry showed that the IgG-adsorbed layers had an optical thickness of 140 A and a surface concentration of 1.7 mg/m2 at low bulk solution concentration — in concentrated solutions the surface amount was 3.4 mg/m2 with a thickness of 320 A (Fig. 17). 

Dalle-Donne I., Rossi R., Giustarini D., Milzani A., and Colombo R. (2003). Protein carbonyl groups as biomarkers of oxidative stress. Clin. Chim. Acta 329 23-38. 

A.R. Hipkiss, Carnosine and protein carbonyl groups a possible relationship. Biochemistry Moscow. 65(2000)907-916. 

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