Protein binding site determination

Phaneuf MD. Szycher M, Berceli SA, Dempsey DJ, Quist WC, LoGerfo FW. Covalent linkage of recombinant hirudin to a novel ionic poly (carbonated) urethane polymer with protein binding sites determination of surface anti-thrombic activity. Artif. Organs 22 657-665,1998. 

Woods, V.L. Jr. Hamuro, Y High resolution, high-throughput amide deuterium exchange-mass spectrometry (DXMS) determination of protein binding site structure and... 

A measurement system that is able to quantitatively determine the interactions of receptor and G protein has the potential for more detailed testing of ternary complex models. The soluble receptor systems, ([l AR and FPR) described in Section II, allow for the direct and quantitative evaluation of receptor and G protein interactions (Simons et al, 2003, 2004). Soluble receptors allow access to both the extracellular ligandbinding site and the intracellular G protein-binding site of the receptor. As the site densities on the particles are typically lower than those that support rebinding (Goldstein et al, 1989), simple three-dimensional concentrations are appropriate for the components. Thus, by applying molar units for all the reaction components in the definitions listed in Fig. 2A, the units for the equilibrium dissociation constants are molar, not moles per square meter as for membrane-bound receptor interactions. These assemblies are also suitable for kinetic analysis of ternary complex disassembly. 

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