Protein attenuated total reflection, polymer

A less technically complicated approach for tracking the surface dehydration ability of proteins and polymers is by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). Accordingly, a solution of a surface binding solute is pumped into a flow cell with a Zn-Se crystal sensor, where it is irradiated with IR light and reflected to a detector. The surface chemistry and area of the sensor are specified and the surface area increased using different types of nanobeads (50 nm in diameter). 

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