Protein adsorption surface topography

Specific domains of proteins (for example, those mentioned in the section Organic Phase ) adsorbed to biomaterial surfaces interact with select cell membrane receptors (Fig. 8) accessibility of adhesive domains (such as specific amino acid sequences) of select adsorbed proteins may either enhance or inhibit subsequent cell (such as osteoblast) attachment (Schakenraad, 1996). Several studies have provided evidence that properties (such as chemistry, charge, and topography) of biomaterial surfaces dictate select interactions (such as type, concentration, and conformation or bioactivity) of plasma proteins (Sinha and Tuan, 1996 Horbett, 1993 Horbett, 1996 Brunette, 1988 Davies, 1988 Luck et al., 1998 Curtis and Wilkinson, 1997). Albumin has been the protein of choice in protein-adsorption investigations because of availability, low cost (compared to other proteins contained in serum), and, most importantly, well-documented conformation or bioactive structure (Horbett, 1993) recently, however, a number of research groups have started to examine protein (such as fibronectin and vitronectin) interactions with material surfaces that are more pertinent to subsequent cell adhesion (Luck et al., 1998 Degasne et al., 1999 Dalton et al., 1995 Lopes et al., 1999). 

SURFACE PROPERTIES AFFECTING PROTEIN ADSORPTION HYDROPHILICITY/HYDROPHOBICITY TOPOGRAPHY ENERGY CHARGE ETC. 

M.S. Lord, M. Foss, F. Besenbacher, Influence of nanoscale surface topography on protein adsorption and cellular response. Nano Today 5 (2010) 66-78. 

Roach P, Farrar D, Perry CC. Surface tailoring for controlled protein adsorption effect of topography at the nanometer scale and chemistry. J Am Chem Soc 2006 128 3939 5. 

Cai K, Bossert J, Jandt KD. Does the nanometer scale topography of titanium influence protein adsorption and cell proliferation Colloid Surface B 2006 49 136 4. 

Zheng J, Song W, Huang H, Chen H. Protein adsorption and cell adhesion on polyure-thane/Pluronic surface with lotus leaf-hke topography. Colloids Surf B Biointerfaces 2010 77 234-9. http //dx.doi.Org/10.1016/j.colsurfb.2010.01.032. 

The overview of innate immune system activation on material surfaces (see Section 4.6) assumes that the inherent properties of the material remain constant over time and that the response is mainly due to the protein adsorption onto the material surface. However, the situation is more challenging when it comes to biodegradable materials, because they (obviously) will change in surface topography and size over time. In addition, because the degradation of most commonly used materials leads to the production of hydroxyl acids and eventually carbon dioxide, a lowering of the pH in the adjacent tissue is found. This change in pH leads to a dramatic increase in complement activation and opsonization [4], and to increased inflammation. 

Therefore, it is necessary to find an effective way to examine and quantify how the protein adsorption is influenced by geometric topographies on mesoporous Ti02 surfaces with different pore sizes. As shown in Fig. 19, it seemed that the amount of adsorbed protein is related to the pore size of the mesoporous Ti02. But we cannot get any regular information. The mechanism to govern the protein adsorption on the mesoporous... 

Song, W., Chen, H. (2007). Protein adsorption on materials surfaces with nano-topography. Chinese Science Bulletin, 52, 3169-3173. http //dx.doi.org/10.1007/sll434-007-0504-6. 

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