Proteases properties

The function of Jisper Uis fermentation appears to be primarily the breakdown of protein and polysaccharides by secreted proteases and amylases. Replacement oiPispergillis by chemical or enzymatic hydrolysis has no major impact on the organoleptic properties of the sauce. Likewise, inoculation with a pure culture of Ixictobacillus delbrueckii to carry out the acetic acid fermentation produces a normal product. The S. rouxii and Toru/opsis yeasts, however, are specifically required for proper flavor development. 

The use of a bioadhesive, polymeric dosage form for sustained dehvery raises questions about swallowing or aspirating the device. The surface area is small, and patient comfort should be addressed by designing a small (less than 2 cm ), thin (less than 0.1 mm (4 mil) thick) device that conforms to the mucosal surface. The buccal route may prove useful for peptide or protein dehvery because of the absence of protease activity in the sahva. However, the epithelium is relatively tight, based on its electrophysiological properties. An average conductance in the dog is 1 mS/cm (57) as compared to conductances of about 27 and 10 mS/cm in the small intestine and nasal mucosa, respectively (58,59) these may be classified as leaky epitheha. 

Like other proteins, enzymes are potential allergens. In addition, proteases may act as skin and eye irritants. However, during the production and handling of industrial enzymes, the occupational health risks entailed by these properties can be avoided by protective measures, and by the form in which... 

Thompson, J. F., et al. (1997). Mutation of a protease-sensitive region in firefly luciferase alters light emission properties. J. Biol. Chem. 272 18766-18771. 

One exciting approach is the development of short sequences of RNA that bind specifically to HCV helicase and/or the protease activity found in the same hepatitis C virus-encoded non-structural protein, NS3, and inhibit helicase at sub-micromolar concenttations (Umehara et al. 2005). These molecules could provide the basis for developing potent helicase inhibitors with improved pharmacotherapeutic properties. 

---