Protease Aspergillus niger var

Protease (Aspergillus niger var.) Produced by controlled fermentation using Aspergillus niger var. The purified enzyme occurs as an off white to tan, amorphous powder. Soluble in water (the solution is usually light yellow), but practically insoluble in alcohol, in chloroform, and in ether. Major active principle protease. Typical application used in the production of protein hydrolysates. 

Protease (Aspergillus niger var.) Produced by controlled fermentation using Aspergillus niger var. The purified enzyme occurs as an off-white to tan, amorphous powder. Soluble in water... 

Chymosin protease (1) Aspergillus niger var. awamori d-calf prochymosin gene (2) Escherichia coli K-12 d-calf prochymosin gene (3) Kluyveromyces marxianus cleaves a single bond in kappa casein cleaves a single bond in kappa casein 3.4.23.4... 

Application and Principle This procedure is used to determine the proteolytic activity, expressed as hemoglobin units on the tyrosine basis (HUT), of preparations derived from Aspergillus oryzae var. and Aspergillus niger var., and it may be used to determine the activity of other proteases at pH 4.7. The test is based on the 30-min enzymatic hydrolysis of a hemoglobin substrate at pH 4.7 and 40°. Unhydrolyzed substrate is precipitated with trichloroacetic acid and removed by filtration. The quantity of solubilized hemoglobin in the filtrate is determined spectrophotometrically. 

---