Prostate Extracts and Perfused Lungs

RCS as an unstable cyclooxygenase metabolite of arachidonic acid, thromboxane A2 (TXA2) second, the finding that aspirin and other non-steroidal anti-inflammatory drugs exert their effects by inhibiting cyclooxygenase activity—a discovery of momentous therapeutic importance. 

An initial series of studies provided evidence that SRS-A was likely to be an arachidonic acid metabolite produced by a biochemical pathway distinct from cyclooxygenase. This evidence was based on chemically impure preparations of SRS-A, however, and so remained circumstantial until 1979, when the coupling of a relatively new analytical technique (high-performance liquid chromatography, HPLC) with classical bioassay methods allowed Robert C. Murphy, Bengt Samuelsson and their collaborators at the 

when J. Capdevila, J.R. Falck, E.H. Oliw and their collaborators reported that liver cytochrome P450 converts arachidonic acid into a family of novel epoxides,researchers in the 

Rich AM, Weissman G, Anderson C, et al. Calcium-dependent aggregation of marine sponge cells is provoked by leukotriene B4 and inhibited by inhibitors of arachidonic acid oxidation. Biochem Biophys Res Commun 1984 121 863-870. 

Graves SY, Dietz TH. Prostaglandin E2 inhibition of sodium transport in the freshwater mussel. J Exp Biol 1979 210 195-201. 

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