Promoter, xylose-inducible

B. megaterium, in contrast to B. subtilis, has low protease activity. It is able to grow on a wide variety of substrates. Plasmids with xylose-inducible promoter are most frequently used for high-level expression [41]. It was successfully used to express toxins and other difficult proteins where the proteins were intact after secretion into the media. Yields of about 30% of total soluble protein were obtained in some cases [42]. 

After confirmation of the recombinant production of the viral T7 RNA polymerase, the already introduced model protein GFP was used for the comparison of the T7 and the xylose-inducible promoter systems. Therefore, recombinant GFP... 

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