Proflavin sulfate

Diaminoacridine sulfate (proflavin sulfate) [1811-28-5] M 516.6, m >300°(dec), Xmax 456nm. An aqueous soln, after treatment with charcoal, was concentrated, chilled overnight, filtered and the ppte was rinsed with a little diethyl ether. The ppte was dried in air, then overnight in a vacuum oven at 70°. 

Preliminary experiments carried out in this laboratory by L. Eisen-stein and P. Debey have shown that it is possible to stabilize the oxygenated complexes of cytochrome P-450 in supercooled fluid media. However, in order to form these oxygenated compounds the cytochrome P-450 must be reduced either enzymically or chemically. In both cases, this results in the presence of an excess of reducing agents in the sample. The chemical reduction can be obtained by either the addition of an excess of dithionite or photochemically in the presence of acridine orange and methylviologen (Gunsalus et al., 1972) or of proflavine sulfate. 

Sulfate dihydrate. CnHuN OjS, proflavine hemisulfate, neutral proflavine sulfate. Orange-red bitter crystals, hygroscopic. Sol in 300 parts cold water, in l part boiling water slightly Sol in ale insol in ether. CHCI,. pH of satd soln is 6-8. 

C19H24N0O12P C13H12N3 SO4 23 H2O 3 2 Cytidylyl-(3 — 5 )-guanosine-proflavine, sulfate. PFCYGUIO 38 527... 

CidHj NgOijPXC HjjNsh SO o 11.5 H2O Proflavine cytidylyl-(3 — 5 )-guanosine, sulfate, hydrate (PFCYGU10)181... 

C2 Z = 4 Dx = 1.41 R = 0.102 for 4,115 intensities. The structure is a 3 2 complex of proflavine and CpG. The asymmetrical unit contains one CpG molecule, 1.5 proflavine molecules, 0.5 sulfate ion, and 11 5 water molecules. Two CpG molecules form an antiparallel, Watson-Crick, miniature duplex, with a proflavine intercalated between the base pairs through the wide groove. The double helix has exact (crystallographic), two-fold symmetry, and the crystallographic, two-fold axis passes through the C-9-N-10 vector of the intercalated proflavine. A second and a third molecule of proflavine are stacked on top of the C - G pairs ... 

Prolonged extraction was not required when acidified methanol was employed for the extraction of acriflavine and proflavine from catfish tissues (507), when acetonitrile was used for the extraction of methylene blue and four metabolites from milk (508), or when dichloromethane in presence of sodium sulfate was employed for the extraction of leucogentian violet from chicken fat (491-494). 

C77H811N25O28P2S 23 H2O, Proflavine cytidylyl- 3, 5 )-guanosine sulfate hydrate, 45B, 685... 

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