Peptidomic Profiling

Similar to SELDI-TOF MS profiling, magnetic RP beads and Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS have been used for salivary peptidome characterization under pathophysiological conditions, as, for example, in the evaluation of periodontal-orthodontic treatment (32). However, the information retrieved using these strategies is limited requiring complementary bottom-up approaches. 

One major issue underlying salivary peptidome characterization is peptide quantification. While some quantitative methods developed for proteome studies were inherited, peptidome analysis has its own quantification traits. Direct profiling with MALDI-MS or SELDI-MS has been one of the most widely used approaches for peptidome despite its limitations. For instance, Zhang el al. (32,44) used magnetic beads combined with MALDI-MS for saliva profiling in response to orthodontic procedures, solid foods, and teeth... 

Fiedler, G.M., Leichtle, A.B., Kase,)., et al. (2009) Serum peptidome profiling revealed platelet factor 4 as a potential discriminating peptide associated with pancreatic cancer. Clin. Cancer Res., 15 (11), 3812 3819. 

Facdiiano, A., et al. (2010) Standardized sample preparation phases for a quantitative measurement of plasma peptidome profiling by MALDl-TOF. 

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