Production propagation, container plants

Forskolin, a labdane diterpenoid, was isolated from the tuberous roots of Coleus forskohlii Briq. (Lami-aceae) [1], C. forskohlii has been used as an important folk medicine in India. Forskolin was found to be an activator of adenylate cyclase [2], leading to an increase of c-AMP, and now a medicine in India, Germany, and Japan. The production of forskolin is completely dependent on the commercial collection of wild and cultivated plants in India. We have already set up the production of monoclonal antibodies (MAbs) against forskolin [3]. The practical application of enzyme-linked immunosorbent assay (ELISA) for the distribution of forskolin contained in clonally propagated plant organs and the quantitative fluctuation of forsko-lin depend on the age of C. forskohlii [4,5]. As an extension of this approach, we present the production of the immunoaffinity column using anti-forskolin MAb and its application [6]. 

Vincamine (40) is derived from Vinca minor. The in vitro propagation of this plant has been studied (709). Axillary shoot proliferation was best with media containing BAP and NAA. As part of a program for the industrial production of this alkaloid plant cell cultures have been studied as a possible source (710). Petiard and co-workers (711,712) reported the initiation of cell cultures of V. minor based on TLC analysis small amounts of... 

Industrial fermentation plants consist of three main sections preparation, fermentation, and product recovery. The preparation section usually contains operations such us medium preparation and sterilization and inoculum propagation. The fermentation section is the heart of the plant where the transformation of raw material into products takes place. The product recovery section encompasses the downstream operations needed to obtain the product of interest with the required purity (Reisman 1988). In the design of fed-batch and batch fermentation plants, one faces the problem of figuring out the adequate combination of the number and size of fermenters to be used to meet the desired production schedule. In principle, the problem has an infinite number of solutions because for any given fermenter size, a number of units of that size will do the work. Nevertheless, not all solutions are equal from an economic standpoint (Simpson et al. 2005). 

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