Procion Blue

Procion Red M-G Procion Blue M-3G Procion Brilliant Blue M-R... 

Fig. 22.3 Effect of concentration of sodium carbonate (1 5% Procion Blue M-R on viscose rayon in a 30 1 liquor at 20°C)...

With Procion Brilliant Red M-2B, Procion Blue M-3G, and Procion Rubine M-B there is less relative difference because they yield quite heavy dyeings in the absence of urea. 

Procion Yellow M-RS Procion Brilliant Yellow M-6GS Procion Brilliant Orange M-GS Procion Brilliant Red M-2BS Procion Brilliant Red M-5BS Procion Scarlet M-GS Procion Blue M-3GS Procion Brilliant Blue M-RS... 

Dyes (Cibacron Blue, Red, Active Red, Procion Yellow, Procion Blue)... 

FIGURE 1.3 Structures of Cibacron Blue 3GA and Procion Blue, two common binding agents used in dye-ligand affinity chromatography [52]. 

Brown RA, Combridge BS. Binding of hepatitis virus particles to immobilized Procion Blue-HB and Cibacron Blue 3GA. J Virol Meth 1986 14 267-74. 

The concentration of the immobilized ligand should be approximately 8.5 /imol/g dry gel molar absorption coefficient for Procion blue MX-R in glycerol/H20 (1 1, v/v) equals 11 6001/mol/cm. For silica gels the pH should never exceed 8.5 at a maximum temperature of 40°C and even then for a few hours only. For this reason, the performance of the above method is poor when coupling monochlorotriazinyl-dyes whidi... 

Procion blue MX-R is a competitive inhibitor with NAD for lactate dehydrogenase. The immobilized form of this dye is used to purify lactate dehydrogenase from a crude... 

Figure 2. Purification of rabbit muscle L-lactate dehydrogenase (LDH) on TSK GSOOOPW-Procion Blue MX-R. HPAC column, TSK GSOOOPW-Ptocioo blue MX-R (13 /ond/g dry gfft. 5.0 cm x 0.45 cm sample applied, 1 ml, 100 units LX>H, 12.3 rag protein flow-rate, I ml/niin startii solvent, 0.05 M sodium phosphate buffer (pH 7.0) eluant, linear gradient of NaQ (10 ml 0—4 M) in buffer. CoUect 2 mlfractioiis. f — ) enzyme activity, (0-0) protein -42go, (—) NaCI gradient.

Glucokinase may be used for assay of glucose and glycerokinase for assay of serum triglycerides. Both enzymes could be purified by immobilised nucleoside phosphates but this is prohibitively expensive on the large scale. Both proteins bind to Procion Blue MX-3G while Procion Brown H-3R could be used to separate glycerokinase... 

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