Processing body staining

This process seems to reflect gradual increases in the intensity and density of labelled receptor cell bodies and their axons, followed by regional bulbar staining as synaptogenesis proceeds. However, a more extensive range of lectins needs to be examined before the implications of species differences in the membrane glycoproteins can be satisfactorily interpreted (Salazar and Quinteiro, 1998). 

The process of infection of lupine nodule cells by Rhizobia was examined by the thin-section electron microscopic technique, as well as the freeze-fracture technique. Different membranes such as infection thread membranes, peribacterioid membranes, plasma membranes, membranes of cytoplasmic vesicles, and membranes of the Golgi bodies and ER were stained with uranium-lead, silver, phosphotungstic acid, and ZIO (31). ZIO stained the membranes of the proximal face of the Golgi bodies and endoplasmic reticulum. ZIO staining has given good contrast in thick sections such as a cotyledon cell, a root cell, and an aleurone layer for ER, dictyosomes cisternae, mitochondria, and nuclear envelopes (17,32-37). 

Figure 9.38 Concentration profile of copper and zinc in the scanned area of interest measured by LA-ICP-MS on brain samples (hippocampus). Calibration was performed via synthetic matrix matched laboratory standards for 1, 5 and 10 fxgg-1 of analyte (see inserted figures on left). Bottom histologically processed brain tissue in which cell bodies were stained (cresyl violet staining) in order to demonstrate the layered structure of the analyzed region. (]. S. Becker, M. Zoriy, C. Pickhardt, N. Palomero-Gallagher and K. Zilles, Anal. Chem., 77, 3208 (2005). Reproduced by permission of American Chemical Society.)...

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