Processing and Analysis of the NMR Data

Use the reference data and the characteristic carbohydrate NMR parameters (section 6.3.3) to determine the stereochemistry at the individual ring carbons, and try to determine the connectivity among the individual monomers. 

Load the raw data of the selective pseudo 2D H H NOE experiment D NMRDATA OLIGOSAC 1D H OHNO 2D 001001.SER. Decompose the data matrix into a series of 1D FIDs, calculate the difference FIDs and process the whole series following the procedures as outlined in previous chapters. Exploit the options for automatic and serial processing for this purpose. Use the multiple display option to compare the individual NOE spectra and plot the 

Hint Use the multiple display option and display each of the NOE (ROE) difference spectra together with the ID TOCSY spectra of the individual monomer units to recognize and assign intra- and inter-residue NOEs (ROEs) respectively. Use different colours to differentiate between the spin systems of the individual monomers and use a special colour to mark NOEs among different monomers on your hardcopy. Cross check your findings with the conclusions extracted from previous data and carefully check the consistency of your H assignments in all the spectra. 

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