Procedures Employed in Separation

Fractionation op Liver by Cohn, Minot, and Associates 1. Procedures Employed in Separation 

In 1927, Cohn, Minot, and their collaborators (15) attacked the problem of the isolation of the substance or substances in liver effective in the treatment of pernicious anemia. Their general procedure was to divide raw minced beef liver into various portions such as  

They followed the course of the active principle by noting the presence or absence of a reticulocyte response after the administration of an extract to a pernicious anemia patient during relapse. At first, the material was given orally, but later in the work, it was administered intravenously. In case of negative clinical results, the responsiveness of the patient was tested with liver or liver extracts of proved potency. 

The procedures employed in the separation of the several fractions are presented schematically below (Table I). [The first part of the diagram is 

Alcohol-Soluble Fractions F) [Removes about 30% of solids of (D)j 

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