Procedures Are the Most Versatile and Productive Purification Methods

Column Procedures Are the Most Versatile and Productive Purification Methods 

Column procedures are the most effective and most varied of purification methods. Common to all col- umn procedures is the use of a glass cylinder with an 

Many variations on this basic procedure are in common use  

Ion-exchange chromatography makes use of the fact that proteins differ enormously in their affinity for positively or negatively charged columns. However, the cross-linked resins used in amino acid fractionation (see chap- 

Collecting fractions during column chromatography. Column material and elution procedure are chosen to effect optimal separation of the desired protein. 

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Column Procedures Are the Most Versatile and Productive Purification Methods Electrophoresis Is Used for Resolving Mixtures Sedimentation and Diffusion Are Used for Size and Shape Determination Protein Purification Procedures... 

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