Prions of Ure2p and Sup35p, Respectively

Further work showed that residues 1-80 were an even more efficient inducer of [URE3] appearance than 1-65 (Maddelein and Wickner, 

1999) and that other parts of the Ure2 molecule could influence the efficiency of this process. Deletion of residues 221-227 results in inability of 

Remarkably, two nonoverlapping prion-inducing fragments can be made from Ure2p. Although the Ure2p region is inactive in indue- 

Native Ure2p isolated from yeast is a dimer (Taylor et al., 1999), as is that produced in Escherichia coli (Perrett et al., 1999). Chemical denatu-ration studies of a series of deletion mutants showed that the absence of the prion domain did not affect the overall stability of the Ure2p molecule (Perrett et al, 1999). This indicates that any energy of interaction of the C-terminal and N-terminal domains is equivalent to that of alternative C-terminal - C-terminal interations. 

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