Primary transgene

Despite the complexity of the experiments and the enormous data manipulation necessary, complex biological pathways, as well as new drug targets are being identified by this method. Examples include screens for compounds that arrest cells in mitosis, that block cell migration, and that block the secretory pathway [50], or assays with primary T cells from PLP TCR transgenic mice for their inhibitory activity on the proliferation and secretion of proinflammatory cytokines in PLP-reactive T cells [51], and identification of small-molecule inhibitors of histone acetyltransferase activity [52]. 

Dent, L.A., Daly, C.M., Mayrhofer, G., Zimmerman, T., Hallett, A., Bignold, L.P., Creaney, J. and Parsons, J.C. (1999) Interleukin-5 transgenic mice show enhanced resistance to primary infections with Nippostrongylus brasiliensis but not primary infections with Toxocara cams. Infection and Immunity 67, 989-993. 

Segregation Simple crossing procedure Requires independent cointegration of primary and marker transgenes 37... 

---