Presequences target peptides

Several additional lines of evidence indicate that presequence function is governed by conformational properties of the precursor. The target peptide of yeast mitochondrial cytochrome oxidase subunit IV (COX), fused to murine dihydrofolate reductase (DHFR, a cytoplasmic enzyme). 

Roise, D and Maduke, M. (1994) Import of a Mitochondrial Presequence into P. Denitrificans, FEBS Letters, 337, 9-13 Cavalier-Smith, T. (1987) The Simultaneous Symbiotic Origin of Mitochondria, Chloroplasts and Microbodies, Annals of the New York Academy of Science, 503, 55-71 Cavalier-Smith, T. (1992) The Number of Symbiotic Origins of Organelles, BioSystems, 28, 91-106 Hartl, F Ostermann, J., Guiard, B and Neupert, W. (1987) Successive Translocation into and out of the Mitochondrial Matrix Targeting of Proteins to the Inner Membrane Space by a Bipartite Signal Peptide, Cell, 51,1027-1037. 

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