Preparing and Resolving Complex Specimens

Another completely different strategy for increasing the dynamic range of proteins is by removing the highly expressed proteins to improve detection of those expressed at lower levels. Within a given sample, individual protein concentrations often span many orders of magnitudes, with most of the 

A different study purely focused on isolating carrier molecules and their bound proteins to search for biomarkers of clinical interest. It was not only found that circulating carrier proteins were reservoirs for the accumulation and amplification of putative disease markers but also that the low molecular mass proteins that bound to albumin were distinct from those bound to nonalbumin carriers. Using SELDI-TOF, it was further verified that albumin bound peptides associated with ovarian cancer. This demonstrated that albumin capture was an effective method for harvesting disease-relevant biomarkers [66]. 

---