Preparative Chromatography in Biotechnology

In biotechnology several different preparative chromatographic separation techniques are common, the most common ones being ion-exchange chro- 

RPLC is the most common technique for small organic molecules. It is also a powerful tool for preparative peptide separations, but is less common for protein purification, because it is often associated with protein denatura-tion. Nevertheless, there are successful examples of large-scale protein purification by RPLC [99], In this thesis the substances used and produced in the biotechnological synthesis, in papers I and II, were small molecules (Mw 1000) and therefore belong to a sample class for which RPLC is the dominant separation technique. 

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Cramer SM, Jayaraman G. Preparative chromatography in biotechnology. Curr Opin Biotech 1993 4 217-25. 

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