Preparation of NOC template in Schramm-Hestrin SH medium

The never-dried NOC template was put into Schramm-Hestrin (SH) medium (Hestrin and Schramm 1954) at pH 6.0, and the solvent was exchanged and finally maintained in a Petri dish until used for the study. 

The author thanks Professor R.M. Brown, Jr. at University of Texas, Austin for the research collaboration and his valuable comment. Mr. Togawa and Ms. Hishrikawa at FFPRI are acknowledged by the long-term research collaboration. Dr. W. Kasai and Ms. Y. Tomita in my laboratory at Kyushu University are also acknowledged by their valuable comment on the text. This research was supported partly by a Grant-in-Aid for Scientific Research (No. 14360101), Japan Society for the Promotion of Science (JSPS). 

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