Preparation of Ergotamine and Ergocryptine

Preparation of Ergotamine and Ergocryptine Alba-Maria Amici Anacieto Minghetti Tollio Scotti Celestino Spalla Milan, Italy July 17,1968 

According to the invention, a strain of Claviceps purpurea FI 32/ 17 is cultivated under aerobic conditions in submerged culture in a 

1) Preparation of the aqueous potato extract 200 g. of peeled potatoes are cut into pieces and boiled for 45 minutes in 500 cc. of tap water. The mixture is filtered through gauze and taken up to the initial volume. 

From a stock of cultures on slants of C+ medium (Table 1) an inoculum is made on a slant of the same medium. The inoculum is incubated at 28° C for 8 days and the culture thus obtained is employed to inoculate two 300 cc flasks, each containing 50 cc of the following SC medium  

The flasks are incubated for 4 days at 24° C on a rotary shaker at 220 r.p.m. with a stroke of 3.5 cm. The cultures thus obtained are used, in the amount of 10%, to inoculate 300 cc flasks containing 50 cc of the following 668 medium  

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