Preparation of Drug Extracts for Analysis

Powdered, drug (1 g is extracted by heating on a water bath for 10 min with 5 m) methanol 20. i,l of the hltrate is used for TLC investigation. 

Powdered drug (1 g) is moistened with about 1 ml 10% ammonia solutions ml methanol is added and the drug is then extracted for 10 min on a water bath 20 and 100 ii of the filtrate are used for TLC analyses. 

A methanolic extract is prepared according to method described for anthraglycosides. The extract is evaporated to about 1 ml, mixed with 0.5 ml water and then extracted with 3 ml n-butanol (saturated with water) 20 pi and 100 [il of the butanol phase are used for TLC investigation. 

Powdered drug (1 g) is mixed with 5 ml 50% methanol and lOnil 10% lead (II) acetate solution and then heated for 10 min on the water bath. The filtrate is cooled to room temperature and then extracted twice with 10-ml quantities of dichloromethane. The combined DCM extracts are evaporated and the residue is dissolved in DCM-methanol (1 1) 100 f.d of this solution is used for TLC investigation. 

Powdered drug (1 g) is extracted by heating under reflux for ISniin with 10 ml DCM. T he filtrate is evaporated to dryne.ss, and the residue is dissolved in 0.5 ml toluene 20- 

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