Pregnanediol, urinary, method

Cooper et al. (10) and Cox (11) were the first to report the gas chromatographic analysis of urinary pregnanediol. These methods normally involved hydrolysis, extraction, and the chromatographic analysis of the free pregnanediol, the acetate derivative, the trimethylsilyl ether derivative, or the bis-heptafluor-obutyrate derivative. [Pg.507]

The traditional method for the determination of urinary pregnanediol has generally been that of Klopper et al. (9). [Pg.506]

Acid may be used to hydrolyze urinary pregnanediol conjugates in a reproducible manner with approximately 90% recovery. The overall advantage to this method is the amount of time saved. Acid hydrolysis normally takes about 15 min enzyme hydrolysis takes 24 hr. Since many steroid molecules can be broken by acid hydrolysis, enzyme hydrolysis is the preferred method. [Pg.508]

The presence, in the sample, of more than one glucuronic conjugate in any appreciable amount calls for a preliminary separation. The numerous methods suggested for determination of urinary pregnanediol glucosiduronic acid will not be reviewed. [Pg.201]

Fig. 2.2. Flowdiagram of the method for the simultaneous determination of urinary androstanediols and pregnanediols.

For some ten years the most useful method for measuring urinary pregnanediol was that of Klopper et al. (K8). The principal isomer excreted in human urine is the 5j8(H)-3a,20a -diol. Much smaller quantities of the 5a (H) epimer and of the 5a(H)-3j8-OH bis-epimer have been... [Pg.124]

Sulimovici, S., Lunenfeld, B., and Shelesnyak, M. C., A practical method for estimation of urinary pregnanediol and allopegnanediol using thin layer chromatography. Acta Endocrinol. 49, 97-106 (1965). [Pg.138]

Darcey and Evenson (D4) have compared GLC and the Zimmerman reaction in the analysis of total 17-ketosteroids in urine. Urinary 17-keto-steroid GLC patterns have been studied by Cawley et al. (C5), who noted the presence of several peaks with retention times shorter than those of 17-ketosteroids. A comparison of different methods of hydrolysis has been made by Curtius and Muller (C18) in their study of the GLC of 17-ketosteroids and progesterone metabolites of urine. The simultaneous determination by GLC of pregnanediol and pregnanolone in urinary extracts has been reported by Guarnieri and Barry (G8). GLC was found to be the method of choice in a study by Barry et al. (B2) of four methods for the quantitative analysis of pregnanediol in urine. [Pg.240]

Separation of Urinary Androstanediol and Pregnanediol Isomers by a Combined Gas-Liquid Chromatography-Thin Layer Chromatography Method J. Chromatogr. 93(1) 149-165 (1974) ... [Pg.21]

Routine Gas-Liquid Chromatographic Method for Simultaneous Assay of Major Urinary 17-Ketosteroids, and Pregnanediol and Pregnanetriol... [Pg.113]

Rapid Gas-Liquid Chromatographic Method for the Quantitation of Urinary Estrone, 17B-Estradiol, Estriol, and Pregnanediol Throughout Pregnancy... [Pg.132]

A Modified Method for the Analysis of Urinary 17-Keto Steroids, Pregnanediol and Pregnanetriol by Gas-Liquid Chromatography in Normal Subjects and Subjects with Various Endocrine Disorders Acta Endocrinol. (Copenhagen) 54(1) 37-50 (1967) CA 66 52577a... [Pg.210]

The Clinical Application of a Gas Chromatographic Method for the Routine Determination of Urinary Pregnanediol... [Pg.227]

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