Precautions to be taken when using virus infected cells

Precautions to be taken when using virus-infected cells 

Viruses produce CPEs on cells and are the agents for many diseases in humans and other animals. In addition, many viruses (e.g. oncoma viruses, herpes type II, adenovirus and polyoma and SV40) are believed to be agents responsible for tumour formation in animals. Moreover, due to their ability to pass through bacteriological filters it is difficult to exclude viruses from uninfected cell cultures if the viruses are present in suspension in the air of the culture room. For these reasons it is recommended practice to take special precautions when using viruses. 

Group Nucleic acid Average MW xlO6 Virion diam. (nm) Shape Symmetry env. eg- 

Corona RNA ss 3 80-120 Roughly spherical Helical + Murine hepatitis 

Rhabdo Arena RNA RNA ss ss 6 175x 75 85-120 Bullet shaped Roughly spherical Helical Helical + + VSV Lassa 

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