Pre-lab 7.2.b Introduction to Solid-Phase Peptide

Pre-lab 7.2.b Introduction to Solid-Phase Peptide Synthesis (SPPS)11 12 

Solid-phase synthesis of peptides was pioneered by Bruce Merrifield in the early 1960s. This work, for which he won the Nobel Prize in 1984, set in motion the modern approach to drug discovery called combinatorial chemistry. Through combinatorial chemistry, millions of compounds are generated by the synthesis of libraries on solid supports and screened for therapeutic activity by high-throughput assays. The importance of this work is attested by the numerous combinatorial research units that are now an integral part of most major pharmaceutical companies. 

The general reagents and apparatus for SPPS are given in Truran et al.13 

Preparation of Resin The amounts used are based on the Fmoc-Gly-Wang resin, having a loading capacity of 0.75 meqg-1 and an average amino acid MM of 500 g mol-1. 

Add 250 mg of Fmoc-Gly-Wang resin to a 10 cm3 syringe barrel that has been fitted with a polypropylene filter. 

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