Practical Considerations in the Design of Solid Phase FI Optosensing Systems

2 Practical Considerations in the Design of Solid Phase FI Optosensing Systems 

Techniques and manifolds used for other on-line soiption separation methods described in the previous sections may be used to advantage for solid phase optosensing systems. 

A flow-cell/column packed with C 8 used by Ruzicka et al.[60,61] for the determination of phosphate by absorptiometry is shown in Fig. 4.12. The light was transmitted radially through the column, which had an i.d. of only 2 mm, at a level where the bulk of the analyte was retained. 

Owing to light attenuation in such systems, the light source of the spectrophotometer may have to be modified to provide higher intensities. With double-beam spectrophotometers equipped with photomultipliers, normally the detection system should be sensitive enough for such applications, however, the reference beam will have to be attenuated by a suitable means to balance the two beams. 

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