Practical Application of Deconvolution

As an alternative to the current situation that the use of the deconvolution is not included in any chromatography data system currently available in the market, recently a software tool became available, called hi skim. With hi skim, an imported chromatogram with overlaid peaks can be integrated, using mathematical models. This means, for the separation or analysis of overlaid peaks, deconvolution is averted. With hi skim, the analyst receives a report in the usual way by integrating with the respective interesting peak parameters for further processing. 

The adjustment can be checked and corrected by means of zoom. When the process is finished, the respective peak parameters are reported. 

The chromatograms of the individual peaks can be exported and re-imported to assist in the evaluation in a chromatography data system. Helpful for evaluating the chromatography data system (CDS) is an overlay of imported single peaks for the evaluation and application of separation methods. 

Furthermore, the resulting peak parameters can be helpful for the optimization of a methods, for example, the peak symmetry of overlaid peaks is reality closer determined and not distorted on the separation methods. 

The lot method leads by tailing peaks, as in the area rules described by Meyer [1], always to a loose in area for the first peak [(the 6,7-dehydro-EE-peak here)]. This is the case here. Reducing the concentration and thus the peak area or the peak height results in an increase of the deviation when using the method of Lot. 

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