PPARa target genes

Cherkaoui-Malki, M., K. Meyer, W.-Q. Cao, N. Latruffe, A.V. Yeldandi, M.S. Rao, C.A. Bradfield and J.K. Reddy. Identification of novel peroxisome proliferator-activated receptor a (PPARa) target genes in mouse liver using cDNA microarray analysis. Gene Express. 9 291-304, 2001. 

To investigate the role of PPARa in the control of cellular lipid homeostasis in vivo, we analyzed the expression of known PPARa target genes encoding mitochondrial and extra-mitochondrial FA oxidation enzymes in response to a major perturbation in FA flux caused by pharmacologic inhibition of mitochondrial import of LCFA by etomoxir. The results obtained showed that the expression of peroxisomal acyl-CoA oxidase (AGO) gene was induced in heart and liver of mice receiving a five day course of etomoxir (Fig. 1). CPT 1 inhibition by etomoxir also led to a marked increase in the expression of... 

Analysis of the expression of PPARa target genes in the liver (Fig. 2) and heart (data not shown) of PPARa -/- female survivors demonstrated that etomoxir did not induce the gene e q)ression, further supporting a role of this nuclear receptor in response to a perturbation of cellular lipid homeostasis. 

Ringseis, R., A. Muschick, and K. Eder. 2007b. Dietary oxidized fat prevents ethanol-induced triacylglycerol accumulation and increases expression of PPARa target genes in rat liver. Journal of Nutrition 137 77-83. 

---