Potential Problems when Performing Kinetic Resolution

2 Potential Problems when Performing Kinetic Resolution 

When taking samples for the determination of enantiomeric purity in a kinetic resolution, it is mandatory to choose the proper time window, e. g., when conversion is between 20% and 80%. If the process of directed evolution leads to enzyme variants differing widely in activity, mass screening needs to be time-resolved, i.e., several samples of a given reaction should be taken as a function of time. 

Jacobsen, A. Pfaltz, H. Yamamoto, Comprehensive Asymmetric Catalysis, Vol. I-m, Springer, Berlin, 1999. b) R. Noyori, Asymmetric Catalysis in Organic Synthesis, Wiley, New York, 1994. c) K. D. Shimizu, M. L. Snapper, A. H. Hoveyda, Chem. Eur. J. 1998,4, 1885-1889. 

Cornils, W. A. Herrmann, Applied Homogeneous Catalysis with Organometallic Compounds, Vol. 1-2, VCH-Wiley, Weinheim, 1996. 

Seelbach, C. Wandrey, Industrial Biotransformations, V CH-Wiley, Weinheim, 2000. 

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