Industrial biotransformations

A. Liese, K. Seelbach, C. Wandrey, Industrial Biotransformations, Wiley-VCH, Weinheim, 2000. 

These first examples illustrate the importance of a sufficient separation of products and byproducts, whereas membranes are one possibility in pharmaceutical production to obtain this aim. Therefore, they are one key tool to obtaining better quality products and environmentally friendly processes. For a more detailed article about the state of the art of membranes in biotechnology, see Rios et al. [27]. At the same time, it can be seen that stoichiometric cofactor need is no longer a limitation for industrial biotransformations, since they can be overcome with efficient recyclization methods. 

Figure 4.23 Industrial biotransformations points of application for reaction engineering...

Vanillin (4-hydroxy-3-methoxybenzaldehyde) is widely used in foods, beverages, perfumes and the pharmaceuticals industries. Biotransformation of isoeugenol from essential oil to vanillin represents an economic route for the supply of vanillin, which has a limited supply due to the availability of vanilli pod plants. The conversion yield of isoeugenol to vanillin by the whole-cell biotransformation process of Bacillus fusiformis was low due to the product inhibition effect. Adding resin HD-8 to the whole-cell biotransformation eliminated the product inhibition effect, yielding 8 gL 1 of vanillin in the final reaction mixture [27]. The resin HD-8 also facilitated the separation of vanillin from the used substrate. The recovered isoeugenol can be used for the subsequent biotransformation reaction. 

Straathof, A.J. (2006) Quantitative analysis of industrial biotransformations, in Industrial Biotransformations, 2nd edn (eds A. Liese, K. Seelbach and C. Wandrey), Wiley-VCH Verlag GmbH, pp. 515-520. 

Tao J, Pettman A, Liese A (2006) Chapter in industrial biotransformations. Wiley-VCH, Weinhein, Germany, pp 63-91... 

For an industrial biotransformation, it is often necessary to further optimize an appropriate biocatalyst. This includes the elimination of the follow-up enzymes in the wild-type strain by mutations and the improvement of other characteristics by additional mutations and the selection of improved strains. Alternatively, the genetic information for a desired enzyme might be introduced in a host that has many of the preceding characteristics, and that has no enzymes that could modify or degrade the desired product. 

Figure 1.5 Enzyme Types Used in Industrial Biotransformations (based on 134 processes). (Reprinted from Straathof, A.J.J., Panke, S. and Schmid, A. The production of fine chemicals by biotransformations. Curr. Opin. Biotechnol. 2002,13, 548-556 with permission from Elsevier.)...

Liese A, Seelbach K, Wandrey C (2000) Industrial biotransformation. WUey-VCH, Weinheim... 

Liese, A., Seelbach, K., Wandrey, C. Industrial Biotransformations, Wiley, VCH Weinheim, 2000. 

Rao N, Liitz S, Seelbach K, Liese A (2006) In Liese A (ed) Industrial biotransformations, 2nd completely revised and extended edition. Wiley-VCH, Weinheim, pp 115-145... 

Industrial Biotransformations, Wiley-VCH Verlag GmbH, Weinheim. 

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