Postgrafting method

New avenues could come from reacting small chemical entities with the remaining amines at the surface of the lipoplexes, to obtain completely neutralized DNA-loaded particles (57). Gain in circulation time was significant by this so-called postgrafting method. 

The second limitation, however, remains i.e., lipoplex stabilization, which prevents DNA release. A combination of solutions should be envisioned for further improvement. For instance, we could combine the postgrafting method with exchangeable PEG. We indeed combined the use of acid-sensitive PEG-lipid and the postgrafting method. Results tend to show an improved DNA release cumulated with higher circulation time (unpublished). However, the differences in tumor growth and vascularization render difficult the obten-tion of significant and reproducible results. 

Two synthetic methods have been exploited to incorporate azobenzene moieties into mesoporous silica frameworks. The postgrafting method involves functionalizing mesoporous sol-gel materials with reactive azobenzene derivatives to tether azobenzene moieties to sol-gel matrices. The one-pot synthesis method requires... 

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