Polysaccharides noncovalent complexes

Cellulose microfibrils make up the basic framework of the primary wall of young plant cells (3), where they form a complex network with other polysaccharides. The linking polysaccharides include hemicellulose, which is a mixture of predominantly neutral heterogly-cans (xylans, xyloglucans, arabinogalactans, etc.). Hemicellulose associates with the cellulose fibrils via noncovalent interactions. These complexes are connected by neutral and acidic pectins, which typically contain galac-turonic acid. Finally, a collagen-related protein, extensin, is also involved in the formation of primary walls. 

In order to avoid misleading spots on the 2-DE profile and to remove salts, lipids, polysaccharides, or nucleic acids interfering with separation, samples should be solubilized. Solubilization procedure involves disruption of all noncovalently bond protein complexes into a solution of polypeptides. It is the most critical step of 2-DE. 

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