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Polynucleotide probes

Fig. 1. Light-directed synthesis of polynucleotide probe arrays. Exposure to light through a photolithographic mask is used to remove protecting groups from surface sites in pre-defined regions of a functionalized glass substrate. A solution of an activated polynucleotide building block is then applied, which reacts specifically in the exposed regions of the substrate. Repeated cycles of illumination-deprotection and monomer coupling are used to construct a two-dimensional array of probe sequences... Fig. 1. Light-directed synthesis of polynucleotide probe arrays. Exposure to light through a photolithographic mask is used to remove protecting groups from surface sites in pre-defined regions of a functionalized glass substrate. A solution of an activated polynucleotide building block is then applied, which reacts specifically in the exposed regions of the substrate. Repeated cycles of illumination-deprotection and monomer coupling are used to construct a two-dimensional array of probe sequences...
Table 1. Combinatorial synthesis of polynucleotide probe arrays... Table 1. Combinatorial synthesis of polynucleotide probe arrays...
Pernthaler, A., Preston, C. M., Pernthaler, J., DeLong, E. F., and Amann, R. (2002b). Comparison of fluorescently labeled oligonucleotide and polynucleotide probes for the detection of pelagic marine bacteria and archaea. Appl. Environ. Microbiol. 68, 661-667. [Pg.378]

A wide variety of hybridization conditions can be expected for this heterogeneous group of solid phases. The hybridization time depends on the nature of the solid phase and complexity of the strand in excess and may range from that comparable to membrane hybridization (Section 8.2 e.g., microtiter plates and polynucleotide probes), to a few minutes (small particulate solid phase will display a hybridization rate which approaches that of solution hybridization Table 3.16). [Pg.163]

Platinum complexes probes of polynucleotide structure and antitumour drugs. S. J. Lippard, Acc. Chem. Res., 1978,11, 211-217 (56). [Pg.53]

McGhee JD, von Hippel PH. Formaldehyde as a probe of DNA structure. 3. Equilibrium denaturation of DNA and synthetic polynucleotides. Biochemistry 1977 16 3267-3275. [Pg.66]

Langer, P.R., Waldrop, A.A., and Ward, D.C. (1981) Enzymatic synthesis of biotin-labeled polynucleotides Novel nucleic acid affinity probes. Proc. Natl. Acad. Sci. USA 78, 6633-6637. [Pg.1086]

J.J. Storhoff, R. Elghanian, R.C. Mucic, C.A. Mirkin, and R.L. Letsinger, One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. J. Am. Chem. Soc. 120, 1959-1964 (1998). [Pg.478]

The S P R of Au N Ps has also been applied to nanobiotechnology and Au N Ps probes can be used for the colorimetric detection of oligonucleotides [185], polynucleotides with single base imperfections [186], antigens [187] or adenosine [188]. [Pg.170]

Polymetaphosphate 3 Polymorphonuclear leukocytes 26 Polynucleotide(s) 199s, 226-228. See also Nucleic acids, RNA, DNA hybridization 255 hydrazine reactions 254 interactions with ions 218 melting of 255 probes 255... [Pg.929]

V. Brabec and G. Dry hurst, Electrochemical behaviour of natural and biosynthetic polynucleotides at the pyrolytic graphite electrode. A new probe for studies of polynucleotide structure and reactions, J. Electroanal. Chem., 89 (1978) 161-173. [Pg.434]

Engel, J. D., and von Hippel, P. H. (1978). D(M6ATP) as a probe of the fidelity of base incorporation into polynucleotides by Escherichia coli DNA polymerase I. J. Biol. Chem. 253, 935-939. [Pg.433]


See other pages where Polynucleotide probes is mentioned: [Pg.198]    [Pg.255]    [Pg.117]    [Pg.118]    [Pg.120]    [Pg.198]    [Pg.255]    [Pg.428]    [Pg.22]    [Pg.291]    [Pg.269]    [Pg.625]    [Pg.29]    [Pg.270]    [Pg.198]    [Pg.255]    [Pg.117]    [Pg.118]    [Pg.120]    [Pg.198]    [Pg.255]    [Pg.428]    [Pg.22]    [Pg.291]    [Pg.269]    [Pg.625]    [Pg.29]    [Pg.270]    [Pg.1164]    [Pg.1164]    [Pg.374]    [Pg.183]    [Pg.411]    [Pg.66]    [Pg.472]    [Pg.12]    [Pg.139]    [Pg.657]    [Pg.980]    [Pg.384]    [Pg.466]    [Pg.468]    [Pg.469]    [Pg.255]    [Pg.257]    [Pg.1293]    [Pg.1171]    [Pg.979]    [Pg.75]   
See also in sourсe #XX -- [ Pg.255 ]

See also in sourсe #XX -- [ Pg.255 ]

See also in sourсe #XX -- [ Pg.255 ]

See also in sourсe #XX -- [ Pg.255 ]




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Polynucleotide

Polynucleotides

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