Polymerase chain reaction split primers

Fig. 2. Direct transcriptional template generation from cDNA library by using the split-primers polymerase chain reaction (PCR) technique. (a-c) A schematic representation of the split-primers design for equipping the cDNA sequences with required UTRs, where b and c are expected PCR-generated DNAs and mRNA, respectively, (cl) Split-primer PCR-generated products.

Fig. 5. (Opposite page) Polymerase chain reaction (PCR)-based expression of cDNA information using conventional method (A) and a new strategy using split-type primers (B). a. Design of the split-type primers for the introduction of the required UTRs into cDNA sequences, b and c. Expected PCR-generated DNAs and mRNA, respectively, d. PCR-generated DNA. e. A 10-pL aliquot from aPCR sample was used forthe 100-pL transcription reaction, and transcripts were analyzed, f. All of the transcript was used for batch-mode translation (50 pL, 4 h), and products were analyzed by autoradiography. (From ref. 36a.)...

Modern DNA profiling is based on the characterization of short tandem repeats (STRs) that are regions (loci) on the chromosome that repeat at least twice within the DNA. For profiling, the number of repeats at each location on the chromosome is determined. To do this, the DNA is first amplified via the polymerase chain reaction (PGR), in which the double-stranded DNA is split into two single strands and a mixture of enzymes and primers are used to replicate specific STR regions of the DNA. In the United States, STRs at thirteen loci are typically considered. The reaction is repeated many times, generating exact copies of the STRs. Because of this... 

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