Polyethylene glycol stock

To measure the pH, remove 5-mL aliquots from the stock of colloidal gold, and add two drops of 1% polyethylene glycol. Do not add the PEG to the colloidal gold stock. Discard aliquots after reading pH. 

Monoclonal antibodies are most commonly produced by employing the hybridoma technique. Figure 2 illustrates this technique. In this method, splenocytes (normal B-cells) from an immunized animal are fused with myeloma cells (tumor B-cells) using a fusion medium such as polyethylene glycol (PEG). Stock myeloma cell lines selected for the fusion process are specifically chosen because they are derived from a common ancestor MOPC-21 known to have lost the ability to produce IgG, so that the only antibody produced is derived from the antigen-sensitized splenocyte. The resultant hybrid cell is known as a hybridoma and inherits antigen specificity from the splenocytes and immortality from the myeloma cells, thus creating a... 

Polyester wax. For 500 g stock, melt 480 g of 400-polyethylene glycol distearate (PEGD) (Aldrich Chemical Co.) at dO C overnight Melt 20 g cetyl alcohol (Merck, Poole, UK) at 60°C. Warm up the PEGD to bO C, add the cetyl alcohol, mix well, pour into convenient size trays, and allow to set Store at d C. 

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