Polyadenylation sequence

Figure 39-19. Structure of a typical eukaryotic mRNA showing elements that are involved in regulating mRNA stability. The typical eukaryotic mRNA has a 5 noncoding sequence (5 NCS), a coding region, and a 3 NCS. All are capped at the 5 end, and most have a polyadenylate sequence at the 3 end. The 5 cap and 3 poly(A) tail protect the mRNA against exonuclease attack. Stem-loop structures in the 5 and 3 NCS, features in the coding sequence, and the AU-rich region in the 3 NCS are thought to play roles in mRNA stability.

The cDNAs are about 2 kb in length and contain a single open reading frame encoding 383 amino acids, followed by a 600 bp 3 -untranslated sequence. This 3 -untranslated region contains two functional polyadenylation sequences (AAUAAA), which are both used, as demonstrated by RACE-PCR. 

Specific inhibition of templated biosynthesis by electroneutral polynucleotide analogs can be achieved even with complex templates Messenger RNA coding for globin, similarly to other messenger RNA molecules, contains a polyadenylate sequence located... 

Plasmid pACCMVplpASR(-) (the shuttle vector ) contains the heterologous CMV promoter, a polylinker, and SV40 polyadenylation sequences flanked by... 

Plasmid vectors are composed of multiple elements as shown in Figure 3.1. These components typically include a strong promoter located upstream of the 5 ATG start codon for the DNA coding for a foreign gene of interest and a polyadenylation sequence (poly A tail) located downstream Irom the 3 stop codon... 

Bergmann, I. E., and Brawerman, G., 1977, Control of breakdown of polyadenylate sequence in mammalian polyribosomes Role of poly (adenylic acid)-protein interactions, Biochemistry 16 259. 

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