Poly mRNA electrophoresis

Northern and Southern blot analyses are carried out as shown in Figure 2.7 and as described below. Restriction enzyme digested DNA (Southern) or total RNA or poly(A) mRNA (Northern) is subjected to agarose gel electrophoresis, which separates DNA and RNA molecules based on their size. The separated molecules are then transferred to a nylon or nitrocellulose membrane by capillary action transfer... 

Purther evidence that cellular mRNA is not degraded after infection came from experiments using cellular mRNA extracted from infected cells to prime protein synthesis in extracts. From mouse plasmacytoma tumor cells Lawrence and Thach (26) isolated a poly(A)-containing 10S RNA fraction which in extracts was translated into a protein found in plasmacytoma cells, as determined by co-electrophoresis and tryptic mapping. This messenger RNA, when isolated from infected cells, was less active in translation than when isolated from uninfected cells by about However, mRNA... 

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