Poly immunofluorescent staining

Figure 25. A-D Immunofluorescence staining of vmculrn in vascular smooth muscle cells on day 3 after seeding on polymeric surfaces (medium supplemented with 10% fetal bovine serum). A poly(DL-lactic acid), PDLLA B block copolymer of poly(DL-lactic acid) and poly (ethylene oxide) (PEO), PDLLA-6-PEO C, E PDLLA-6-PEO with 5% GRGDSG-PEO-6-PDLLA D, F PDLLA-6-PEO with 20% GRGDSG-PEO-6-PDLLA. E, F Immunoperoxidase staining of bromodeoxyuridine (arrows) incorporated into DNA newly synthesized in vascular smooth muscle cells cultured for 3 days in serum-free medium on PDLLA-Z)-PEO with 5% (E) or 20% (F) GRGDSG-PEO-6-PDLLA. Cells counterstained with light green. Bar=100 pm [41].

Initially, we cultured the control neurons without cryopreservation. Neurons cultured on a poly-L-lysine-coated 24-well plate were examined by microscopy and immunofluorescence staining using MAP2 (Figures 1,2). Arrows indicate living neurons. 

Immunofluorescent Staining of Poly(ADP-Ribose) in Situ in Acetone-Fixed HeLa Cells 497... 

Change of the Immunofluorescent Staining Pattern of Poly(ADP-Ribose) After Incubation with NAD... 

Kanai Y, Tanuma S, Sugimura T (1981) Immunofluorescent staining of poly(ADP-ribose) in situ in HeLa cell chromosome in the M phase. Proc Natl Acad Sci USA 78 2801-2904... 

Fig. 2. Immunofluorescent detection of autoantibodies to poly(ADP-ribose) polymerase. Acetone-fixed HEp-2 cells reacted with a serum containing antibodies to poly(ADP-ribose) polymerase followed by fluorescein-conjugated goat anti-human IgG. The diffuse nuclear staining with nucleolar accentuation is apparent.

In this method, samples of freshly excised tissues were fixed by boiling them in a 10% neutral formaldehyde solution for 5 min. The fixed tissues were then embedded in paraffin and cut into 3 pm thick sections. The sections on glass slides were deparaffinized and dehydrated by passage through xylene and a graded alcohol series. Then they were incubated with a primary antibody against poly(ADP-ribose). The antibody used, IgG monoclonal antibody lOH, recognized the linear structure of poly(ADP-ribose) described previously (3). Bound antibody was stained with die avidin biotin peroxidase complex or by an indirect immunofluorescence technique. 

Figure 10.6 Immunofluorescence images of mesenchymal stem cells (MSCs) cultured on (a) nanofibrous poly(E-caprolactone) scaffolds and (b) tissue culture polystyrene plate showing different cytoskeletal organization (actin and nucleus were stained with Phalloidin and 4, 6-diamidmo-2-phenylindole [DAPI]).

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