Poly compounds with Polynucleotides

Cationic model compounds of polynucleotide such as 62 and 63 were prepared by the Menschutkin reaction of poly-4-vinylpyridine with 9-(2-chloroethyl)adenine and l-(2-chloroethyOthymine80, M>. 

Schuchmann MN, Naumov S, Schuchmann H-P, von Sonntag J, von Sonntag C (2005) 4-Amino-3Ff-pyrimidin-2-one ("isocytosine") is a short-lived non-radical intermediate formed in the pulse radiolysis of cytosine in aqueous solution. Radiat Phys Chem 72 243-250 Schulte-Frohlinde D, Hildenbrand K (1989) Electron spin resonance studies of the reactions of OH and SO4 radicals with DNA, polynucleotides and single base model compounds. In Minisci F (ed) Free radicals in synthesis and biology. Kluwer, Dordrecht, pp 335-359 Schulte-Frohlinde D, Behrens G, Onal A (1986) Lifetime of peroxyl radicals of poly(U), poly(A) and single- and double-stranded DNA and the rate of their reaction with thiols. Int J Radiat Biol 50 103-110... 

Among the synthetic interferon inducers described (polycarboxylates, polynucleotides, low molecular weight compounds), polynucleotides and more specifically double-stranded polyribonucleotides such as poly(I) poly(C) are efficient interferon inducers in vitro (cell cultures). Therefore, studies on the mechanism of interferon production have almost exclusively been performed with ds—RNAs (or viruses). 

In order to prove that one compound specifically interacts with guanine and adenine bases, some experiments can be performed by using S3mthetic polynucleotides of guanine (poly[G] and adenine (poly[Al) [53],... 

Most of the compounds mentioned above are electrically neutral and therefore poorly soluble in water at neutral pH. There are exceptions, such as poly (9-vinyladenine), and poly(l-vinyluracil). All compounds are stable to chemical and enzymatic hydrolysis, and they form complexes with complementary polynucleotides. However, these complexes do not have the simple stoichiometry found in natural nucleic acids. Stability and stoichiometry of complexes do depend on solvent, temperature, and pH of the system (36). Again, because of the complexity of the field, the reader is referred to the review articles mentioned above (36-38). Poly(A -vinyl) derivatives have been carefully tested for their biological and antiviral activity by Pitha (38) in enzyme assays, cell cultures, and in organisms. 

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