Polarization studies as a function of sucrose at

Source Tuma, J. J. (1983). Flandbook of Physical Calculations, McGraw-Hill, New York. 

Prepare a sucrose solution at 60%. Dissolve 12 g of sucrose in 10 ml of buffer by heating and mixing. Once the sucrose is completely dissolved, complete with buffer up to 20 ml. 

Add 165 p of the sucrose solution to 835 /xl of the protein solution the result is a solution with 10% sucrose. The volume of the solution is 1 ml. Measure the polarization of the solution. 

Plot the Perrin plot. What type of plot do you obtain and what information do you obtain  

The slope at high T/rj values gives a (p of 18 ns, a value in the same range (20 ns) of that calculated theoretically. The other slope yields a 4 r of 72 ps this value characterizes the free rotation of a fluorophore and which is generally found to be equal to 100 ps. 

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