Platination of Nucleotides and DNA

DNA has been considered as the major cellular target of platinum anticancer drugs. This idea comes from the numerous experiments on cisplatin showing that the percentage of platinum atoms bound per molecules is the highest for DNA [36,68]. It is not yet clear what determines the adduct profile, but Pt(II) binding to nucleobases is kinetically controlled and the bifunctional adducts are believed to be stable on the time scale of days up to weeks. 

Following and determining the rates of the manifold reactions that occur in the formation of even a single adduct are difficult tasks. Using [ H, N] 1D/2D HSQC to monitor the reaction between N-labeled platinum complexes and nucleobases or DNA is particularly attractive because species can be acquired quickly (in as little as 3-4 min) and all platinated species are observed at micromolar concentrations. The interaction between N-dsplatin 

---